ftidc  (Bio-Techne corporation)

Journal: bioRxiv

Article Title: Aryl hydrocarbon receptor (AHR) and IL-13 signaling crosstalk in human keratinocytes and atopic dermatitis

doi: 10.1101/2023.06.17.545291

Figure Lengend Snippet: Using RNA sequencing analysis of HaCaT cells, we compared the differentially expressed genes (DEGs) from IL-13-treatment (CTL vs IL-13 [10 ng/mL] for 24 hours) to DEGs from IL-13+FICZ treatment (IL-13 [10 ng/mL] vs IL-13 [10 ng/mL] + FICZ [1 µM]) to model AD vs AD+AHR activation. A. Diagram showing differentially expressed genes regulated by IL-13 treatment alone (CTL vs IL-13, 308 genes) as well as a subgroup of these genes which were also regulated by AHR (IL-13 vs IL-13+FICZ, 36 genes, 10.5% of IL-13-regulated genes). B. Heat map of the log2-transformed fold change of the 36 shared genes in (A), sorted first by genes that changed direction (blue box, 24/36 genes, 2/3 of genes), followed by fold change (high to low) in the IL-13 group.). C. Gene Ontology relationships of the 36 shared genes were analyzed.

Article Snippet: Supernatants from HaCaT cells were collected, centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat. No. DY346, Minneapolis, MN, USA).

Techniques: RNA Sequencing Assay, Activation Assay, Transformation Assay

Journal: bioRxiv

Article Title: Aryl hydrocarbon receptor (AHR) and IL-13 signaling crosstalk in human keratinocytes and atopic dermatitis

doi: 10.1101/2023.06.17.545291

Figure Lengend Snippet: RNA sequencing was performed using confluent HaCaT cells exposed to IL-13 (10 ng/mL) with and without FICZ (1 uM) for 24 hours. Genes were limited to those with transcript per million (TPM) >8, and only genes whose absolute fold change was >1.5 and adjusted p-value <0.05 (when comparing any 2 of the 3 groups). To better visualize patterns of expression, columns were arranged to show Control, IL-13 and IL-13+FICZ as follows: A. Genes which were upregulated by IL-13 and downregulated by addition of FICZ. B. Low expression genes which were downregulated by IL-13 and upregulated by addition of FICZ. C. High expression genes which were downregulated by IL-13 and upregulated by addition of FICZ. D. Principal Component Analysis (PCA) of gene expression showing first two principal components that clearly discern between each treatment group.

Article Snippet: Supernatants from HaCaT cells were collected, centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat. No. DY346, Minneapolis, MN, USA).

Techniques: RNA Sequencing Assay, Expressing

Journal: bioRxiv

Article Title: Aryl hydrocarbon receptor (AHR) and IL-13 signaling crosstalk in human keratinocytes and atopic dermatitis

doi: 10.1101/2023.06.17.545291

Figure Lengend Snippet: A. CYP1A1 expression in HaCaT cells treated with AHR endogenous ligand FICZ (1 µM) (Tapinarof 1 µM in [ B. ]), and/or GNF351 (1 µM, AHR blocker) (n=6). C. CCL26 expression in HaCaT cells treated with FICZ (1 µM) (Tapinarof 1 µM in [ D. ]) (n=6). E. CCL26 protein level in HaCaT cells treated with FICZ (1 µM) (Tapinarof 1 µM in [ F. ]) (n=4). One-Way ANOVA with Tukey’s correction for multiple comparisons; * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001.

Article Snippet: Supernatants from HaCaT cells were collected, centrifuged at 4°C for 5 min at 5000 g and middle layers collected and stored at −80°C until analysis using Human CCL26/Eotaxin-3 DuoSet ELISA from R&D Systems/Bio-Techne (Cat. No. DY346, Minneapolis, MN, USA).

Techniques: Expressing

Journal: Dentistry Journal

Article Title: Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity

doi: 10.3390/dj10030038

Figure Lengend Snippet: Immunohistochemical reactivity of neural markers in GCTs and Schwannomas. Immunoreactivity was detected by DAB (brown colour). Images were taken using 200× original magnification. Relative size shown by 100 µm scale bar. ( A ): S100 protein in GCT. ( B ): S100 protein in Schwannoma. ( C ): NSE in GCT. ( D ): NSE in Schwannoma. ( E ): SOX10 in GCT. ( F ): SOX10 Schwannoma. ( G ): GAP43 in GCT. ( H ): GAP43 in Schwannoma.

Article Snippet: GAP43 , Bio-Techne Canada, Oakville On, NB300-143 , Rabbit polyclonal , Regenerating neural tissues/growth cones, GAP43 intracellular growth protein/membrane protein , 1/5000.

Techniques: Immunohistochemical staining

Journal: Dentistry Journal

Article Title: Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity

doi: 10.3390/dj10030038

Figure Lengend Snippet: Mean DAB stain intensity and percentage of cells staining in GCTs and schwannomas.

Article Snippet: GAP43 , Bio-Techne Canada, Oakville On, NB300-143 , Rabbit polyclonal , Regenerating neural tissues/growth cones, GAP43 intracellular growth protein/membrane protein , 1/5000.

Techniques: Staining

Journal: Dentistry Journal

Article Title: Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity

doi: 10.3390/dj10030038

Figure Lengend Snippet: H-scores: H-score for GCTs and schwannomas for anti-GAP43 antibody. ( A ): Statistical comparison using Student’s t -test ( α = 0.0125, p = 0.0108). ( B ): Statistical analysis using Welch’s t -test correction ( α = 0.0125, p = 0.0201); # p ≤ 0.0125. H-score for GCTs and schwannomas for anti-HLA-DR antibody. ( C ): Statistical comparison using Student’s t -test ( α = 0.0125, p = 0.646). ( D ): Statistical analysis using Welch’s t -test correction ( α = 0.0125, p = 0.646). H-score for GCTs and schwannomas for anti-CD163 antibody. ( E ): Statistical comparison using Student’s t -test ( α = 0.0125, p = 0.0012). ( F ): Statistical analysis using Welch’s t -test correction ( α = 0.0125, p = 0.0062); # p ≤ 0.0125, ## p ≤ 0.005. H-score for GCTs and schwannomas for anti-CD68 antibody. ( G ): Statistical comparison using Student’s t -test ( α = 0.0125, p < 0.0001). ( H ): Statistical analysis using Welch’s t -test correction ( α = 0.0125, p = 0.0006); ### p ≤ 0.001.

Article Snippet: GAP43 , Bio-Techne Canada, Oakville On, NB300-143 , Rabbit polyclonal , Regenerating neural tissues/growth cones, GAP43 intracellular growth protein/membrane protein , 1/5000.

Techniques: Comparison

Journal: Dentistry Journal

Article Title: Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity

doi: 10.3390/dj10030038

Figure Lengend Snippet: Summary of the comparison of cell stain intensity using manual semiquantitative scoring and H-score derived from QuPath.

Article Snippet: GAP43 , Bio-Techne Canada, Oakville On, NB300-143 , Rabbit polyclonal , Regenerating neural tissues/growth cones, GAP43 intracellular growth protein/membrane protein , 1/5000.

Techniques: Comparison, Staining, Derivative Assay

Journal: Dentistry Journal

Article Title: Investigation of the Molecular Profile of Granular Cell Tumours and Schwannomas of the Oral Cavity

doi: 10.3390/dj10030038

Figure Lengend Snippet: Summary of the antibodies used for immunohistochemistry of GCTs and schwannomas.

Article Snippet: GAP43 , Bio-Techne Canada, Oakville On, NB300-143 , Rabbit polyclonal , Regenerating neural tissues/growth cones, GAP43 intracellular growth protein/membrane protein , 1/5000.

Techniques: Immunohistochemistry, Cell Surface Receptor Assay, Membrane, Plasmid Preparation